SOME PROPERTIES OF THE AUTOINTERFERING VIRUS RECOVERED FROM THE GASTRIC CONTENT OF PATIENTS WITH EPIDEMIC HEPATITIS
Abstract
From the gastric content of patients with epidemic hepatitis an autointerfering virus was isolated which was capable of reproducing in passages on transplanted cultures of Detroit-6, HEp-2, KEM, and in 9-12 day old chick embryos. The virus isolated possesses autointerfering properties; its cytopathic activity was manifested during dilution of virus-containing culture fluid to 10 to the minus 7th power--10 to the minus 10th power. In cultures of transplanted cells of Detroit-6, HEp-2, HeLa, KEM, Changa, and L, infected with the AIF-virus, a substance of the interferon or inhibitor type was revealed which suppressed the cytopathic effect of the virus. It is destroyed by heating up to 56-60 deg for 30 minutes, and under the influence of 0.1% solution of pepsin and 0.025% solution of trypsin. The antivirus activity of the substance is manifested in homologous tissue culture and is absent in heterologous. Based on these properties the substance revealed is close to interferon. The capacity of AIF-virus to be cultivated in transplanted tissue cultures and chick embryos, its autointerfering properties, high resistance to heating, absence of hemagglutinating activity, and other properties pointed out in the work distinguish it from the known enteric and respiratory viruses.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 1966
- Accession Number
- AD0690317
Entities
People
- D. K. Fomin
Organizations
- United States Army Biological Warfare Laboratories