MORPHOLOGICAL CORRELATIONS INVOLVED IN THE DIFFERENTIATION OF THE EYE LENS.

Abstract

On the basis of immunological techniques, cellulose acetate and starch gel electrophoresis, differences in macromolecular composition of the lens cells were detected in correlation with the age and cytological differentiation. From the three major groups of soluble lens proteins; alpha, beta, and delta crystallins; the last fraction should apparently be considered as a transient antigen. In fact, delta crystallin progressively disappears from the equatorial and cortical cells in relation to aging. Furthermore DC was detected at a higher level in the embryonic core of the organ, and in the epithelial cells after experimental lens rotations. Other dynamic changes of soluble crystallins, suggest that a differential macromolecular biosynthesis exhists during aging and cytological differentiation of the lens. Biochemical and immunological analysis of the lens in the chick, during development, provides qualitative data of genetic expression during the ontogenic differentiation of the organ. This information could be compared with those obtained under experimental conditions in order to understand the mechanism involved in gene regulation of the lens. (Author)

Document Details

Document Type
Technical Report
Publication Date
May 15, 1969
Accession Number
AD0691011

Entities

People

  • Jose Ma Genis-galvez

Organizations

  • University of Granada

Tags

DTIC Thesaurus Topics

  • Acetates
  • Anabolism
  • Cells
  • Cellulose
  • Cellulose Acetates
  • Electrophoresis
  • Epithelial Cells
  • Gel Electrophoresis
  • Regulations
  • Rotation

Fields of Study

  • Biology

Readers

  • Circadian Sleep-Wake Regulation and Chronobiology
  • Molecular Biology and Genetics
  • Molecular and Cellular Biochemistry

Technology Areas

  • Biotechnology
  • Biotechnology - Cancer Biotech