AFLATOXINS IN MAMMALIAN CELL CULTURES
Abstract
KB and HeLa mammalian tissue culture cells were cultured in the presence of 0.4, 1, and 4 ppm of aflatoxin. The incorporation of labeled uridine into the different RNA components separated by sucrose gradient ultracentrifugation and by methylated albumin column chromatography was studied. All levels of aflatoxin increased the proportion of incorporation into RNA in the 4s region after 4 hours and 12 hours of exposure. The longer the time of exposure the more severe was the effect. It is not known whether this is the result of depolymerization or of stimulation of t-RNA synthesis or both.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 1968
- Accession Number
- AD0691294
Entities
People
- Ronald A. Chung