DIFFUSIONAL PHENOMENA IN FLOWING POLYMER SOLUTIONS. I. THEORY OF RESOLUTION DISPERSION IN GEL PERMEATION CHROMATOGRAPHY.

Abstract

A systems approach is applied to the analysis of chromatogram resolution dispersion, or zone broadening, in Gel Permeation Chromatography (G. P. C.). Three possible sources of dispersion are considered; these are: the packed columns, the empty tubing between pump and columns, columns and detector, etc., and the detection system, vis., the differential refractometer cell. It is shown that empty tubing can contribute significantly to the degree of dispersion and to skewness of elution curves and that this dispersion should depend on molecular weight of solute (polymer) and diameter and length of the tubing. The importance of dispersion in the empty tubing is compared with that in the packed columns and refractometer cell. (Author)

Document Details

Document Type
Technical Report
Publication Date
Jul 29, 1969
Accession Number
AD0691466

Entities

People

  • A. Ouano
  • J. A. Biesenberger

Organizations

  • Stevens Institute of Technology

Tags

DTIC Thesaurus Topics

  • Chromatography
  • Detection
  • Detectors
  • Diameters
  • Dispersions
  • Gel Permeation Chromatography
  • Molecular Weight
  • Refractometers
  • Skewness
  • Systems Approach

Readers

  • Aerosol Science/Aerosol Physics
  • Electrical Engineering
  • Groundwater Contamination Remediation.