FUNCTION OF SIALIDASE IN INFLUENZA VIRUSES.

Abstract

In order to investigate the function of sialidase during influenza virus multiplication, methods were developed to isolate and purify the enzyme. With purified enzyme it was possible to characterize the enzyme and to determine the role of the enzyme. Among the properties studied the antigenicity of sialidases from myxoviruses best characterized the enzyme. This was accomplished by preparing highly specific enzyme antisera. The specific enzyme antiserum provided us with the critical reagent by which we demonstrated that the function of the enzyme facilitates the release of virus from infected cells. This was shown by in vitro biological assays and morphological examination by electron microscopy. (Author)

Document Details

Document Type
Technical Report
Publication Date
Sep 01, 1969
Accession Number
AD0693214

Entities

People

  • Joseph T. Seto

Organizations

  • California State University, Los Angeles

Tags

DTIC Thesaurus Topics

  • Assays
  • Bioassay
  • Electron Microscopy
  • Electrons
  • Immune Serums
  • Influenza
  • Microscopy
  • Viruses

Fields of Study

  • Biology

Readers

  • Molecular and Cellular Biochemistry
  • Virology (or Medical Virology).

Technology Areas

  • Microelectronics