PATHWAYS OF GLUCOSE CATABOLISM IN MAMMALS. I. RATE OF CATABOLIC PATHWAYS IN RATS
Abstract
Experiments have shown that with an infusion of 14C-labeled glucoses, steady state metabolism can be controlled. Further, it has been shown that there is minimum endogenous dilution. Methods have been developed for estimating the relative concurrent activity of three primary pathways of glucose catabolism in intact rats. The calculation of this information is based on direct measurement of the catabolic rate of intravenously infused, variously labeled glucoses. Fasted rats were given 1.5 gm unlabeled glucose by stomach tube followed 6 hours later by an infusion of 14C glucose at a rate of 150 mg/hr for 10 hours. Estimated rates of glucoses processed through the pathways was very nearly equal to the rate of infused glucose. Catabolism accounted for 82% while 18% of the infused glucose entered anabolic channels. Participation of the three pathways was 83% for the EMP-Krebs cycle, 11% through the pentose phosphate sequence, and 6% for the glucuronic acid pathway. The understanding of the participation of the various pathways in the rat makes it possible, for the first time, to study the effects of propellant toxicants on these pathways with more assurance that the results will be reproducible.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 1969
- Accession Number
- AD0695481
Entities
People
- C. H. Wang
- D. Johnson
- F. N. Dost
- J. R. Prince
Organizations
- Oregon State University