FLUORESCENT DYES AS A MEANS FOR REVEALING ELECTRIC CHARGES ON THE SURFACE AND INSIDE MICROBIAL CELLS
Abstract
This article reviews the results of luminescence-microscopic observations, confirming the electrostatic mechanism of interaction of acridine orange and uranin with live microbial cells. The luminescence-microscopic observations confirmed that microbial cells carry a negative charge on their surface. However, among the negatively charged cells, cells which are charged positively are detected regularly. The magnitude of the negative charge on cells also is not the same, since with the cation dye AO they are stained not only green, but also a red color. On cells of a cylindrical and ovoid form reinforced electric charges are concentrated on the ends. The value and sign of charge in intracellular structures are apparently variable. For example, usually the spores of yeast cells did not sorb uranin, but in some individuals they luminesced a golden-green. In preparations which were vitally stained with AO the spores inside one ascus often had a different color - two spores gave red and two green fluorescence.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 02, 1969
- Accession Number
- AD0695573
Entities
People
- A. P. Kononenko
- K. I. Kononenko
Organizations
- United States Army Biological Warfare Laboratories