PRESERVING VIBRIOS BY LYOPHILIZATION
Abstract
For the purpose of studying the possibility of prolonged preservation strains of vibrios under laboratory conditions a check was made of the lyophilic drying of four strains of vibrios; ten media were tested as protective ingredients. After combining with the protective media the cultures of vibrios were frozen for 8 hours at minus 50 degrees and then dried for 24 hours under a vacuum from 100 to 50 microns with a temperature of from minus 10 degrees to plus 25 degrees in the drying chamber. An analysis of the results shows that amniotic fluid of cattle and sheep and also medium No 3 turned out to be unsuitable as protective media for the drying of vibrios. Strains of vibrios were preserved for 24 months in the 5% gelatose with 10% saccharose, amino peptide-2, separated milk, and 1.5% gelating with 10% saccharose media. The cultural-morphological, biochemical, and pathogenic properties of the dried strains remained at the initial level.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 02, 1969
- Accession Number
- AD0695574
Entities
People
- A. V. Golikov
Organizations
- United States Army Biological Warfare Laboratories