OBTAINING CONCENTRATED NON-INFECTIOUS CULTURAL ANTIGENS FROM VIRUSES OF THE TICK-BORNE ENCEPHALITIS GROUP
Abstract
The purpose of the present work was to obtain non-infectious cultural antigens of viruses which were suitable for use in the HSR (hemagglutination suppression reaction) and the RDPA (reaction of diffusion precipitation in agar) , in the case of tick-borne encephalitis. Conclusions were as follows: Drying of cultural antigens for 48 hours and more by the open method at 37 and 24 degrees causes the inactivation of the infection capacity of tick-borne encephalitis viruses and the concentration of hemagglutinins and precipitinogens. Optimum pH in the HR and the HSR for concentrated antigens equals 6.2 regardless of the strain of virus investigated and the type of tissue culture on which it is incubated. Investigation of the resulting antigens in the HSR with specific immune sera and the sera of reconvalescents, and also in the RDPA with hyperimmune rabbit sera, immune mouse sera, and specific gamma- globulin showed their specificity in these reactions. Storage of dried antigens in a non-diluted state at 37 and 24 degrees for 4 months and following their dilution with borate buffer solution (pH 9.0) and storage for 7 months at 4 degrees did not cause a lowering of titers of hemagglutinins and precipitinogens.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 1969
- Accession Number
- AD0695581
Entities
People
- S. F. Zakirova
- V. S. Kokorev
Organizations
- United States Army Biological Warfare Laboratories