THE POSSIBILITY OF CONTROLLING THE MUTATION PROCESS BY USING CHEMICAL MUTAGENS REACTING PREFERENTIALLY WITH SINGLE-STRAND DNA IN CONJUNCTION WITH LOCAL CHANGES IN THE STATE OF CELLULAR DNA
Abstract
The higher sensitivity of single-strand or denaturated segments of DNA to chemical mutagens can be used to involve discrete genes in the mutation process and obtain limited mutation spectra in conjunction with local denaturation of cellular DNA. In a number of investigations carried out, it was shown that denaturated DNA reacts more intensely than native DNA with specific chemical mutagens. Thus, in the reaction with hydroxylamine, the cytosine loss is 32.6 plus or minus 1.6 in denaturated DNA and only 10.1 plus or minus 3.2% in native DNA; denaturated DNA adds 2.5 -- 5.6 times as much of the chromophoric derivative of nitrogen mustard as native DNA; denaturated DNA also reacts more intensely with dimethyl sulfate.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 29, 1969
- Accession Number
- AD0695583
Entities
People
- R. I. Salganik
Organizations
- United States Army Biological Warfare Laboratories