PRODUCTION OF HERPES SIMPLEX MUTANTS RESISTANT TO DEXTRAN SULFATE
Abstract
The influence of dextran sulfate on the capacity of the herpes simplex virus to form plaques and the possibility of obtaining mutants which are resistant to this inhibitor was studied. Five mutants were isolated which were resistant to dextran sulfate. They were designated with the symbol DSr. The frequency of mutations comprised 8.8 x 10 to the minus 6th power were genetically stable and did not lose their resistance during subsequent passages under a covering which did not contain dextran sulfate. Serological typing of the mutants obtained in the reaction of neutralization of cytopathic action with specific serum showed their identity with the initial strain. In contrast to the initial virus the plaque-forming capacity of DSr-mutants was not suppressed when dextran sulfate was added to the covering in a dose of 1 mg/ml.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 29, 1969
- Accession Number
- AD0695585
Entities
People
- A. B. Germanov
- L. S. Ratushkina
- M. I. Sokolov
Organizations
- United States Army Biological Warfare Laboratories