PHENOTYPIC MANIFESTATIONS OF CERTAIN GENETIC PROPERTIES OF HERPES SIMPLEX VIRUS DEPENDING ON CONDITIONS OF CULTIVATION
Abstract
A study was made of the influence of incubation temperature and type of cell culture on the plaque-forming capacity and resistance to sulphated polysaccharides on the part of various mutants of the L2K5 strain of the herpes virus. Mutants which were thermoresistant and resistant to dextran sulphate, just as the initial strain, on a culture of chick embryo fibroblasts at 28 deg were more sensitive to sulphated polysaccharides than at 37 deg. Sensitivity to these inhibitors on a culture of human embryo fibroblasts was manifested to a greater degree than on a culture of chick embryo fibroblasts. Mutants, unable to form plaques on the latter of these 2 cultures in the absence of DEAE- dextran, were no different phenotypically in plaque-formation than the initial strain, and on a culture of human embryo fibroblasts formed plaques both with the presence of DEAE-dextran in the covering and without it. Mutants, forming 'blotched' plaques on a culture of human embryo fibroblasts, were no different from the initial strain on a culture of chick fibroblasts, causing the formation of hyaline plaques.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 29, 1969
- Accession Number
- AD0695587
Entities
People
- A. B. Germanov
- M. I. Sokolov
Organizations
- United States Army Biological Warfare Laboratories