METHODS OF ISOLATION OF WEAK VIRUS CULTURES OF PASTEURELLA PESTIS
Abstract
This work presents a discussion of the isolation of weak virus strains of the plague virus from native rodents by the following methods: (1) The conventional method of inoculation -- organ imprints. Sections of liver, spleen, lungs and heart were touched to an agar surface and the imprint material was spread by a loop uniformly on the culture medium surface. (2) Sections of these same organs and of two lymph nodes (inguinal and para-aortic) were carefully rubbed into the agar surface. (3) Part of the organs and of the lymph nodes from each animal were placed in a porcelain mortar and triturated. A drop of the suspension, was placed on the agar surface at the edge of the Petri dish. Part of this drop was scattered by a loop for partial streaking in an area equalling approximately one third of the agar, the loop was then plunged into the same drop of suspension and a second one third of the agar surface was streaked. The remaining area of the agar was treated in the same way.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 29, 1969
- Accession Number
- AD0695588
Entities
People
- L. A. Timofeeva
Organizations
- United States Army Biological Warfare Laboratories