AUTOMATED COLUMN CHROMATOGRAPHIC ANALYSIS OF DEACYLATED PHOSPHOLIPIDS

Abstract

A procedure is fully described for isolation and deacylation of phospholipids from serum or tissue. Control experiments are described that ensure maximum yield with minimum degradation. A completely automated system is described for column chromatographic resolution and quantitative analysis of the fractions of a complex mixture of deacylated phospholipids. Control experiments are described that ensure maximum efficiency of chromatographic separation of and maximum sensitivity of analysis of the fractions. Elution profiles for human serum and red cells and for rat liver deacylated phospholipids are shown. All of the seven fractions are identified.

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Document Details

Document Type
Technical Report
Publication Date
Jun 01, 1969
Accession Number
AD0695635

Entities

People

  • Charles R. Wilson
  • James G. Wetmur

Organizations

  • United States Army Aeromedical Research Lab

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Acids
  • Alcohols
  • Biomedical Research
  • Blood
  • Buffers (Chemistry)
  • Cells
  • Chromatographic Analysis
  • Chromatography
  • Flow Rate
  • Hydroxides
  • Identification
  • Materials
  • Molybdates
  • Security
  • Sodium
  • Sodium Hydroxide
  • Sugar Alcohols

Readers

  • Molecular and Cellular Biochemistry
  • Operations Research