EFFECT OF LASER MICRO-IRRADIATION ON ISOLATED CELLS.
Abstract
The work described was concerned principally with an examination of the effects produced by laser irradiation of isolated human blood cells and various types of cultured cells in an effort to study: (1) the types of cellular damage produced by different wavelengths, energies, and types of laser emission; (2) the possibility of destruction of specific cellular organelles, and (3) the reaction of other cells to an individual cell's death. By comparing the thermal effects produced by laser light is stained cells with those induced in a stained model system (albumin), it was found that it is possible to calculate the concentration of the stain in mitochondria. Evaluation of various parameters of laser irradiation (particularly thermal events) has led to the determination of the optimal irradiation times for both the measurement of local stain concentration and the selective destruction of 'infra-stained' mitochondria. The ultraviolet laser has been used in preliminary experiments to demonstrate, at the ultrastructural level, that the morphological lesions produced in chromatin are very different from those observed after conventional UV irradiation, i.e., coagulation rather than 'paling' is induced in the target area. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 1969
- Accession Number
- AD0697837
Entities
People
- Marcel Bessis
Organizations
- Collège de France