Research Program for Alerting, Detection and Identification of Pathogens
Abstract
The objective of this program is to assess the feasibility of rapidly detecting and identifying pathogens when present in small populations. Gas chromatography is the methodology used. It was established that present methods of ether extraction and silylation of tissue culture supernatants and lysates are not conducive to obtaining reproducible results. Use of halogenated organic acids as media supplements offers promise of being a useful technique. Various methods of ether extraction of blood sera for indication of infection proved to be unsuccessful. Variation in incubation and extraction procedures was effective in altering the signature of bacteria. The effectiveness of the head space technique for obtaining microbial signatures was tested.
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 1969
- Accession Number
- AD0698815
Entities
People
- A. W. Phillips
- C. A. Salinis
- E. Schuster
- J. E. Smith
- J. R. Gould
Organizations
- General Electric