THE EFFECT OF LASER ENERGY ON CELLS IN TISSUE CULTURE.
Abstract
Laser power at 2650 A produced cytolysis and inhibition in the migration of leukocytes. It also inhibited the activity of lactic dehydrogenase, and produced a photoproduct of uridylic acid with a reversible absorption change. Injured cells showed a loss of mitotic activity and an increased frequency of nuclear abnormalities. The focused argon microbeam produced visible lesions in nucleoli which showed a reduced uridine incorporation into RNA. Ablation of the nucleolar organizer sites on chromosomes showed a loss of genetic function in organizing a nucleolus. Concentrations of 0.0001 to 0.01 M ADP could mimic the promotion of blood clotting rates, platelet agglutination, and inhibition of cell growth, which were produced by chemicals that are released from ruby laser-traumatized cells. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Dec 03, 1969
- Accession Number
- AD0699383
Entities
People
- Donald E. Rounds