GROWTH OF VENEZUELAN EQUINE ENCEPHALOMYELITIS VIRUS IN HUMAN DIPLOID CELL STRAIN WI-38

Abstract

It was demonstrated that Venezuelan equine encephalomyelitis (VEE) virus replicated in and adapted rapidly to human diploid cell strain WI-38. Peak titers of approximately 10 to the 9.8th power mouse intracerebral 50% lethal doses were obtained at low passage levels in Eagles basal medium supplemented with calf serum. VEE virus replicated poorly in serum-free medium. Propagation of VEE virus was accompanied by the production of hemagglutinin and cytopathogenic effects.

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Document Details

Document Type
Technical Report
Publication Date
Oct 08, 1969
Accession Number
AD0701251

Entities

People

  • Leonard Green
  • Morton Reitman

Tags

Communities of Interest

  • Energy and Power Technologies

DTIC Thesaurus Topics

  • Anti-Bacterial Agents
  • Arbovirus Infections
  • Blood
  • Cells
  • Dilution
  • Diploid Cells
  • Epithelial Cells
  • Gamma Rays
  • Lethal Dosage
  • Monomolecular Films
  • Production
  • Rift Valley Fever
  • Rodents
  • Tissue Culture
  • Vaccines
  • Venezuelan Equine Encephalomyelitis
  • Viruses

Fields of Study

  • Biology

Readers

  • Mathematics or Statistics
  • Virology (or Medical Virology).