CHANGES IN HUMAN ERYTHROCYTE MEMBRANE PROTEINS DURING STORAGE.
Abstract
Fresh and 21-day old human blood samples were used to prepare fresh and out-dated stromas. Pooled fresh and outdated samples were solubilized by butanol or pyridine. These two protein fractions (fresh and outdated) produced different patterns on disc electrophoresis and column chromatography. Charge differences between fresh and outdated proteins were shown to be independent of the sialic acid content of the proteins. Conformational changes between fresh and outdated proteins were seen using 1-anilino-8-naphthalene sulfonate (ANS) as a fluorescent probe. Fresh samples showed greater fluorescent enhancement but less total ANS binding, and lower protein-ANS dissociation constants. These changes may cause a reduction in the strength, or integrity, of the erythrocyte membrane and be a limiting factor in the viability of the human red cell during in vitro storage. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 09, 1970
- Accession Number
- AD0703181
Entities
People
- Donald A. Cooper
- Gerald L. Moore
- Ross S. Antonoff
- Stephen L. Robinson
Organizations
- United States Army Medical Research Laboratory