INFLUENCE OF SURFACE PROPERTIES OF ANTIGENS ON ANTIBODIES.
Abstract
In the original proposal preliminary data was presented showing that sheep red cells coated with polylysyl gelatin (PLG) elicited a higher hemolytic antibody response in mice than control washed cells. Since PLG gives red cells a positive electrophoretic mobility the assumption was made that response was due to the difference between positive and negative red cell surfaces. This has been substantiated in the present work where it has been shown that provided there is enough PLG to give the cells a positive charge the presence of more material does not affect the response. Further, polylysyl albumin and poly ornithyl gelatin give very similar effects. Using control red cell injected animals, it was found that another injection of PLG coated red cells could elicit an early (7-day) or late (60-day) secondary response, with injections of as few as 1000 cells. In both cases, provided the number of cells injected was suitable the PLG-coated cells provoked a higher titer. No RES hyperplasia or stimulation could be demonstrated, neither were the injected materials pyrogenic. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Nov 01, 1969
- Accession Number
- AD0703306
Entities
People
- David J. Wilkins