EFFECT OF HYPERBARIC OXYGEN-NITROGEN MIXTURES ON MICE.
Abstract
Mice were exposed to compression with 3 atm (rel.) of an air O2 mixture containing 27% O2 for 72 hr. They were injected with 1 microcurie of 14C-lysine at the initiation, 24 and 48 hr after compression was started. The cpm and sp act values of the protein of the carcass were determined. The sp act values were 10, 13, and 14% greater than that of the controls in the three groups. This appeared to be related to reduced food intake rather than any specific effect on nitrogen metabolism. Mice were exposed to 23 cycles each consisting of compression at 45 lb/sq in in an air-oxygen mixture containing 27% oxygen for 72 hr followed by 4 days of recovery. The skin was analyzed for salt-soluble, acid-soluble and insoluble collagen, hyaluronic acid, chondroitin sulfate, and fluorogenic material. Growth was less than that of the controls. There was 25% less salt-soluble collagen and 20% more fluorogenic material but the other values did not differ significantly from that on the basis of size. Acid-soluble collagen, extracted from the skins of newly-weaned mice, was exposed to 150 psi of oxygen for eight weeks. An increase in fluorescence at 360 mu activation/450 mu emission in collagenase digested collagen samples was observed after exposure. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 29, 1969
- Accession Number
- AD0703643
Entities
People
- Harry Sobel
Organizations
- University of California, Los Angeles