ENDOGENEOUS MECHANISM OF VASCULAR RESPONSE IN INFLAMMATION, WITH SPECIAL REFERENCE TO BIOLOGIC SIGNIFICANCE OF SPECIFIC PERMEABILITY FACTORS AND THEIR INHIBITORS NEWLY ISOLATED FROM INFLAMED SITES.
Abstract
Increased vascular permeability and leukocyte emigration are important events in inflammation. In the Arthus reaction in rabbits, the time-course of these events was distinctive, suggesting different chemical mediation. The peptide permeability factors isolated from Arthus skin site in rabbit, induced pronounced edema accompanied by dye leakage, but no polymorphonuclear leukocytes (PMN) emigration. The chemotactic factor, isolated from Arthus skin site in rabbit, did not increase vascular permeability, but induced pronounced PMN emigration followed by a widespread infiltration. The factor (named leukoegresin) was highly purified by chromatography; it behaved as a homogenous substance on electrophoresis and ultracentrifugation. The substance was a protein (free of nucleic acid) of molecular weight of about 140,000, and its effect was relatively heat-stable. Its isoelectric point was about 5. Rabbit leukoegresin was found active not only for rabbit PMN but also for PMN of guinea pig and rat. Furthermore, this substance was shown to share at least some of the antigenicity with rabbit IgG on immunodiffusion and immunoelectrophoresis, and the chemotactic activity of leukoegresin disappeared from the fluid phase on addition of anti-IgG and anti-Fc. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Dec 01, 1969
- Accession Number
- AD0703847
Entities
People
- Hideo Hayashi
Organizations
- Kumamoto University