THE ENDOGENOUS METABOLISM OF ANAEROBIC BACTERIA.
Abstract
Studies of the endogenous metabolism and survival of the non-spore-forming anaerobic bacteria Zymomonas anaerobia and Peptococcus prevotii under conditions of starvation have been continued. The nutritional requirements of Z. anaerobia have been further delineated in order that the organism might be grown under defined conditions. Response curves for biotin and lipoic acid, shown to be essential for growth, revealed that their action is not additive and that together they are not so effective as a mixture containing nine additional vitamins. A detailed survey of the amino acid requirements showed that the organism is more exacting than the related Zymomonas mobilis and growth on ammonium salts is very poor. The ATP content of P. prevotii during growth has been studied and the effect of washing cells prior to analysis examined. The ability to ferment serine during prolonged starvation was gradually lost over a period of 48 hrs, correlating with previously observed response of ATP production to pulses of serine. Extracts of P. prevotii contain acetate kinase and phosphotransacetylase and these enzymes have been studied. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Dec 01, 1969
- Accession Number
- AD0703930
Entities
People
- E. A. Dawes
- J. Bexon
- J. G. Morton
- P. J. Large
- S. Murden
Organizations
- University of Hull