ISOLATION OF SNAKE VENOM TOXINS AND STUDY OF THEIR MECHANISM OF ACTION
Abstract
A threefold approach was made to the study of snake venoms: isolation of venom toxins, elucidation of their mode of action, raising anti- venin potency. Attention was directed to phospholipase, hemolytic polypeptide, kinin-releasing enzyme, hemorrhagin, and antihemorrhagin antibody. Methods included exchange chromatography, gel filtration, histochemical, electron microscopical, phospholipid hydrolysis, microsomal ATPase, enzymatic antibody fragmentation. The conclusions are: Naja naja venom contains several phospholipase A iso-enzymes; Echis coloratus venom contains two kinin-releasing enzymes; fibrin deposition in the kidney by Echis carinatus venom is promoted by epsilon aminocaproic acid; Vipera palestinae hemorrhagin impairs blood coagulation and platelet function by its proteolytic activity; Ringhals phospholipase A inhibits microsomal ATPase; lytic peptide of cobra venom activates hydrolysis of membrane phospholipids but not of soluble phospholipids; bivalence of antibody appears significant in hemorrhagin neutralization. It is recommended that further studies in the above areas are carried out. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 1970
- Accession Number
- AD0704734
Entities
People
- Andre De Vries