BEHAVIOR OF COLIPHAGE LAMBDA IN HYBRIDS BETWEEN ESCHERICHIA COLI AND SALMONELLA
Abstract
Salmonella typhosa hybrids able to adsorb lambda were obtained by mating S. typhosa recipients with Escherichia coli K-12 donors. After adsorption of wild-type lambda to these S. typhosa hybrids, no plaques or infective centers could be detected. E. coli K-12 gal(+) genes carried by the defective phage lambda-dg were transduced to S. typhosa hybrids with HFT lysates derived from E. coli heterogenotes. The lysogenic state which resulted in the S. typhosa hybrids after gal(+) transduction differed from that of E. coli. Ability to produce lambda, initially present, was permanently segregated by transductants of the S. typhosa hybrid. S. typhosa lysogens did not lyse upon treatment for phage induction with mitomycin C, ultraviolet light, or heat in the case of thermoinducible lambda. A further difference in the behavior of lambda in Salmonella hybrids was the absence of zygotic induction of the prophage when transferred from E. coli K-12 donors to S. typhosa. A new lambda mutant class, capable of forming plaques on S. typhosa hybrids refractory to wild-type lambda, was isolated at low frequency by plating lambda on S. typhosa hybrid WR4254. Such mutants have been designated as lambda-sx, and a mutant allele of lambda-sx was located between the P and Q genes of the lambda chromosome. The significance of these results in terms of Salmonella species as hosts for lambda is discussed. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 15, 1970
- Accession Number
- AD0707960
Entities
People
- Elisa Penido
- I. R. Ryman
- L. S. Baron
- Stanley Falkow
Organizations
- Walter Reed Army Institute of Research