CELL POTASSIUM BY K39 SPIN ECHO NUCLEAR MAGNETIC RESONANCE,
Abstract
Pulsed nuclear magnetic resonance (NMR) measurements of K39 in solutions and bacteria were made, by the use of a super-conducting magnet with a field of 50,300 gauss, which provides the necessary increase in sensitivity. Transverse relaxation time (T2) of K39 in a 1 M KC1 solution showed a mean value of approximately 0.0008 seconds. T2 of K39 in aqueous solutions increased with increasing concentrations of potassium. Fifty-nine per cent of K39 in packed wet ion exchange resin was invisible to NMR, presumably due to a marked shortening of T2 complexed by the resin. T2 of K39 in the bacterium H. halobium was shortened by at least 50 per cent, which suggests that cell K(+) is not in free solution in liquid water, but may be complexed to fixed charge sites or solvated in semicrystalline water of the cell. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 11, 1970
- Accession Number
- AD0708382
Entities
People
- Freeman W. Cope
- Raymond Damadian
Organizations
- Naval Air Warfare Center Warminster