DISRUPTION OF YEAST MEMBRANES BY METHYLPHENIDATE.
Abstract
Methylphenidate blocked sorbose uptake and loss by yeast spheroplasts and, at higher concentrations, disrupted spheroplasts. At high concentrations methylphenidate also ruptured the membranes of whole yeast cells; sorbose and 280 nm-absorbing materials were lost from the cells, and methylene blue stained them. Intracellular structures were extensively affected as shown by electron micrographs, and evidently were more sensitive to methylphenidate than the external membrane. N-ethylmaleimide and Ca(++) enhanced the rupture of external membranes by methylphenidate. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 17, 1970
- Accession Number
- AD0712969
Entities
People
- Edward Spoerl
Organizations
- United States Army Medical Research Laboratory