DETERMINATION OF ALPHA-TOCOPHEROL IN FREEZE-DRIED FOODS BY A MODIFIED COLORIMETRIC PROCEDURE.
Abstract
A reliably precise and reproducible determination of alpha-tocopherol was achieved by an adaptation of the classic colorimetric determination of alpha-tocopherol by Emmerie and Engel. The method was preceded by the separation of the various tocopherols either by secondary magnesium phosphate column chromatography (as described by Bro-Rasmussen and Hjarde, and Freed) or by Florisil column chromatography according to the method of Dicks-Bushnell. The basic Emmerie-Engel method, despite its disadvantages, is considered the most suitable method for use with oils, foods, and feeding stuffs. Samples of precooked, freeze-dried ground beef, chicken cubes, whole eggs, corn, beans, and peas were analyzed and alpha-tocopherol values consistent with those reported in the literature were obtained. Recovery values achieved with the addition of pure alpha-tocopherol ranged from a minimum recovery of 90.2% to a maximum recovery of 99.1%. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 01, 1970
- Accession Number
- AD0713829
Entities
People
- Emmett J. Stork
- Ramiro P. Villanueva
Organizations
- United States Air Force School of Aerospace Medicine