Mannitol Uptake by Saccharomyces cerevisiae.
Abstract
The uptake of mannitol, a nonmetabolized hexitol, by Saccharomyces cerevisiae was measured. Various parameters examined include: effects of temperature on uptake, inhibition of uptake by uranyl nitrate, competition for uptake by glucose, counterflow of mannitol by glucose, and the affinity of mannitol for a carrier system as measured by a Michaelis constant. That energy is required for uptake was shown by a decreased uptake in the presence of energy inhibitors, by an increased uptake upon addition of energy sources, and by the absence of uptake under anaerobic conditions with no fermentable energy sources available. That mannitol is bound to some cellular constituent after it enters the cell was shown by its attachment to nondialyzable cell fragments and by the lack of an osmotic response, both of which are consistent with a minimal efflux. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 14, 1970
- Accession Number
- AD0714187
Entities
People
- Edward Spoerl
- W. A. Maxwell
Organizations
- United States Army Medical Research Laboratory