A Fast Method of Cattle Brucellosis Diagnosis (Uskorennyi Metod Diagnostiki Brutselleza Krupnogo Rogatogo Skota),

Abstract

As a supplement to RA (Agglutination reaction) and RSK (complement-fixation reaction), Soviet researchers have proposed a number of new and valuable methods of recognizing latent and active forms of brucellosis. The search for faster and simpler test methods, suitable for use on collective farms, state farms and in veterinary medicine installations has been of importance. A method of fast RA with a chromatic antigen for KR on filter paper was tested. This method, in essence is a modification of the Kheddl'son reaction, is very simple to carry out and consists of mixing equal drops of test serum and chromatic antigen on glass and immediately transferring one drop of the mixture to filter paper. After absorption, which has a maximum duration of 1-1.5 min., the reaction is analyzed. If the result is positive, the colorless part of the liquid spreads to the periphery of the filter paper and in its center is formed a dark blue spot. In the case of a dubious reaction the spot in the center is less intensively colored and bluish rays spread out from it. In negative cases a light blue drop spreads uniformly over the entire surface of the paper and the central part is less intensively colored.

Document Details

Document Type
Technical Report
Publication Date
Sep 02, 1970
Accession Number
AD0714923

Entities

People

  • A. I. Smolin
  • G. V. Suvorova
  • P. S. Lazarev

Organizations

  • United States Army Foreign Science and Technology Center

Tags

DTIC Thesaurus Topics

  • Absorption
  • Agglutination
  • Boundaries
  • Brucellosis
  • Filter Paper
  • Filters
  • Paper
  • Test Methods
  • Veterinary Medicine

Readers

  • Immunology
  • Information Retrieval
  • Vision Science/Vision Psychology/Cognitive Neuroscience.