Penetration and Fate of Exogenous DNA into Cells of Normal and Irradiated Mammalian Tissues.
Abstract
A CsC1 gradient analysis of the fate of exogenous labelled DNA in ascites tumour cells reveals that those cells take up the foreign material and integrate it with their own DNA as double stranded molecules bound by covalent linkages to the recipient double stranded DNA. This hetero duplex subsequently becomes replicated by the dividing cells and is transmitted to the progeny. The irradiation of the recipient cells increases the amount of DNA they take up. When bacterial DNA is injected intraperitoneally in mice, large amounts of polymerised exogenous DNA appear in the blood and circulate with it. Foreign DNA does not associate with the red blood cells, but is taken up by living organs and tissues. The injection of high molecular DNA in irradiated mice interacts with the sequence of events which follow irradiation in such a way that the mouse survival is very significantly improved. The restorative effect of a given DNA depends on the mouse strain, the dose-rate and the total dose used. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 1970
- Accession Number
- AD0715018
Entities
People
- Gilbert Mattelin
- Jacques Remy
- Jean-rene Maisin
- Lucien Ledoux
- Pol Charles