Radiation-Induced Ultrastructural Changes in Lysosomes. II. Biochemical Analysis.
Abstract
A biochemical analysis for acid phosphatase was made of soluble protein and lysosomes in liver cells of rats exposed to 2 krads of x rays. The results clearly showed an increase in the acid phosphatase activity in the lysosomes and in soluble protein 2 hours after exposure. This was followed by a decrease in enzyme activity in the lysosomes and a consistent high level in the soluble protein. The results correlated well with earlier cytochemical studies and showed that, in addition to the radiation-induced increase in number and size of lysosomes, there is a distinct increase of acid phosphatase in the lysosomes themselves. This substantiated earlier findings. In addition, the persistent high level of acid phosphatase in the soluble protein (cytoplasm) was a possible indication of early radiation impairment of the lysosomal membrane. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 1970
- Accession Number
- AD0715474
Entities
People
- A. A. Rene
- J. H. Darden
- J. L. Parker
Organizations
- Armed Forces Radiobiology Research Institute