Hemoglobin Function in Stored Blood. VI. The Effect of Phosphate on Red Cell ATP and 2,3-DPG.
Abstract
The objective of the study was to determine if a higher concentration of inorganic phosphate than 2mM (CPD: citrate-phosphate-dextrose) would benefit red cell concentrations of ATP and 2,3-diphosphoglycerate during blood storage. Blood preservatives with phosphate concentrations of 2 mM (CPD), 5 mM, 10mM, 15mM, and 20 mM were used to study red cell preservation under blood bank storage conditions at 4 C. Procedural and analytic methods previously employed in these laboratories were employed. Concentrations of phosphate higher than 2 mM (CPD) were found to be of no benefit for maintenance of ATP and 2,3-DPG during 5 weeks of storage. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Dec 22, 1970
- Accession Number
- AD0719702
Entities
People
- Ben Dawson Jr.
- Walter F. Kocholaty
Organizations
- United States Army Medical Research Laboratory