Odor Sensing Cell Ultrastructure by Electron Microscopy.
Abstract
The research is divided into two main sections, cytological and biochemical. Light and/or electron microscopy was used to make the following determinations on rabbit olfactory cells: (1) The incidence of centriole migration in the sensing cells was highest in the fetus and very young animal. (2) Olfactory tissue was regenerated; the new cellular material was derived from the Bowman's glands. (3) Light microscope observations of odor molecules tagged with radioisotopes indicated that the odor molecules actually entered the olfactory cells. In determining the inhibition of animal tissue ATPase activities by chlorinated hydrocarbon pesticides, four pesticides were tested on homogenates of red blood cells, brain, olfactory, kidney, heart, liver and muscel from rabbits, chickens, lake trout and cockroaches. The pesticides inhibited both the Na(+)-K(=) ATPase and Mg(2+) ATPase enzyme systems differing according to concentrations. In studying the effects of octanol on ATPase activites, comparisons were made between olfactory tissue fractions and similar fractions from brain cortex homogenates. From the response differences of the various fractions to octonol, it appears that the Mg(2+) ATPase of the plasma membrance (and other membrances) is different from the Mg(2=) ATPase of the mitochondria. The Na(=)-K(+) ATPase activities were strongly inhibited in all fractions at the highest level of octonol used. At low levels, however, there was a difference in response between the olfactory and brain fractions. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Dec 01, 1969
- Accession Number
- AD0721224
Entities
People
- Herbert E. Heist
- Robert B. Koch
Organizations
- Honeywell International, Inc.