Growth of Chikungunya Virus in Baby Hamster Kidney Cell (BHK-21-Clone 13) Suspension Cultures
Abstract
The report describes the methods used to obtain high titers of chikungunya virus with suspension culture of BHK-21-clone 13 cells. The cells were grown at 37 C to a cell concentration of one to two million per ml. After maximum cell growth, the cells were inoculated with chikungunya virus at a multiplicity of 1 to 2 50% suckling mouse intracerebral lethal doses SMICLD/50 per cell in the spent Eagle's minimum essential medium for suspension cultures (MEMS), or the cell cultures were centrifuged at 200 X g and resuspended in either fresh MEMS or medium 199 prior to inoculation. The medium used had no effect on virus titer. The inoculated cultures were incubated at 34 C until the cell viability dropped to 30%, which usually occurred 28 to 30 hr postinoculation. After these procedures, chikungunya virus titers of log10 10.3 to 11.8 SMICLD/50 per ml were obtained.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 09, 1970
- Accession Number
- AD0723256
Entities
People
- Howard M. Hodge
- Jack L. Davis
- William E. Campbell Jr.