Acetylcholinesterase Activity in Synaptic Vesicles of the Medulla of Rats Exposed to High Doses of Gamma-Neutron Radiation

Abstract

Sprague-Dawley rats were subjected to a whole-body dose of 15 krads of mixed gamma-neutron radiation. At 2 and 24 hours following irradiation 14 animals from the irradiated group and 14 unirradiated controls were sacrificed by replacing the blood in the brain circulation by perfusion with a sucrose- eserine solution. The medulla section of the brain was removed, homogenized and prepared for ultracentrifugation and sucrose density gradient separation. The separated synaptic vesicles were assayed for protein and acetylcholinesterase (ACHE). A decrease in protein was found 2 hours after irradiation with a greater decrease 22 hours later. A decrease in ACHE activity was also seen 2 hours after irradiation but there was no further decrease at 24 hours. These results were correlated with vascular changes found during the central nervous system (CNS) syndrome.

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Document Details

Document Type
Technical Report
Publication Date
Apr 01, 1971
Accession Number
AD0724295

Entities

People

  • A. A. Rene
  • J. H. Darden
  • J. L. Parker

Organizations

  • Armed Forces Radiobiology Research Institute

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Biological Sciences
  • Biology
  • Blood
  • Blood-Brain Barrier
  • Brain
  • Cardiovascular Physiological Phenomena
  • Cells
  • Central Nervous System
  • Health Services
  • Military Research
  • Navy
  • Nervous System
  • Nuclear Energy
  • Nuclear Medicine
  • Public Health
  • Radiologic Health
  • Synapses

Fields of Study

  • Medicine

Readers

  • Cardiovascular Physiology
  • Neuroscience
  • Nuclear and Radiation Engineering.