Ultrafiltration as a Method for Concentrating Rift Valley Fever Grown in Tissue Culture

Abstract

Filtration by means of a Diaflo ultrafilter was used to concentrate three 1,000-ml lots and one 3,000-ml lot of tissue culture-grown Rift Valley fever virus. Quantitation of both infectivity and total protein was achieved. Water treatment with continued ultrafiltratin of the virus concentrate provided a final virus product approximately 99.25% free of low-molecular-weight materials originally present in the growth medium.

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Document Details

Document Type
Technical Report
Publication Date
Dec 09, 1970
Accession Number
AD0724863

Entities

People

  • Bill G. Mahlandt
  • Frederick Klein
  • Hobert B. Bonner
  • Ralph E. Lincoln

Tags

DTIC Thesaurus Topics

  • Acids
  • Amino Acids
  • Conductivity
  • Filtration
  • Indicator Dyes
  • Materials
  • Membranes
  • Molecular Weight
  • Proteins
  • Resistance
  • Rift Valley Fever
  • Rift Valleys
  • Tissue Culture
  • Tissues
  • Valleys
  • Viruses
  • Vitamin C

Readers

  • Environmental Engineering
  • Virology (or Medical Virology).