Distribution of Arbovirus Antigens in Density Gradients

Abstract

Sucrose gradient analysis of Venezuelan equine encephalitis virus, a group A arbovirus, indicated that hemagglutinating (HA) and complement-fixing (CF) activities were coincident with virus infectivity. In contrast, West Nile, Ilheus, and Rio Bravo viruses of group B were fractionated into infectious and noninfectious components. The infectious component sedimented as a single entity, possessed HA and CF activity, and had a buoyant density of l.19 g/cc. West Nile and Ilheus virus suspensions contained a slower sedimenting noninfectious HA and CF component with a buoyant density of 1.16 g/cc. Rio Bravo virus suspensions did not contain a similar component. A very slow-sedimenting noninfectious CF component (6 S, 1.10-1.12 g/cc) was detected for each of the group B viruses.

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Document Details

Document Type
Technical Report
Publication Date
Nov 13, 1970
Accession Number
AD0726488

Entities

People

  • Jack Gruber
  • Mark F. Stinski

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Anti-Bacterial Agents
  • Antigens
  • Blood
  • Body Fluids
  • Cells
  • Encephalitis
  • Equine Encephalitis
  • Health Services
  • Immune Serums
  • Proteins
  • Public Health
  • Rodents
  • Viruses

Fields of Study

  • Biology

Readers

  • Aerosol Science/Aerosol Physics
  • Virology (or Medical Virology).