The Effect of Laser Energy on Cells in Tissue Culture.
Abstract
The ultraviolet laser (UVL) produced different degrees of effects on cells of different types. Hamster (DON-C) cell populations were reduced in number following treatment, but recovered numerically, morphologically, and in rates of 3H-TdR uptake. Human malignant (CMP) cells did not show recovery. Human lung (WI-38) cells showed loss of nucleolar and ribosomal components, while mitochondria were the most sensitive structure in CMP cells. Multiple exposures caused a reduction in DNA templace activity during RNA synthesis, and it inhibited rates of LDH catalysis. The UVL produced known types of chromosomal aberrations in DON-C cells, but human lymphocytes were primarily killed, but showed no aberrations in survivors. A concentration of 0.1 microgram/ml of quinacrine was not toxic, but could photosensitize nucleoli of CMP cells to the blue wavelengths from the argon laser. Mitochondria of myocardial cells contained natural chromophores, the cytochromes. Acridine orange-stained newt lung chromosomes showed both an 0.5 micron diameter paling and a loss of function following argon microbeam laser treatment. Recent studies showed a relationship between the nucleolar organizer and the mitotic process. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 11, 1971
- Accession Number
- AD0729384
Entities
People
- Donald E. Rounds