Sedimentation Characteristics of Venezuelan Equine Encephalomelitis Virus
Abstract
The VEE virus was propagated in chick embryo cells, concentrated and purified. The optimal method for obtaining biologically active virus components consisted in degradation of the virus with ether-Tween. The purified VEE virus sedimented at about 380 S in sucrose gradients, the nucleoid at about 160 S. Centrifugation in CsCl gradients showed the VEE infectious material to band in two main positions: most of the three viruses banded at 1.25 g/ml., and a smaller amount at 1.42 g/ml. The main peak of hemagglutinins was detected at a boyant density of 1.25 g/ml. The site of virus and its components was determined by radiological and biological tests.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 1970
- Accession Number
- AD0732706
Entities
People
- A. F. Bykovskii
- F. I. Ershov
- L. V. Uryvaev.
- V. M. Zhdonov
- Yu. V. Chernetsov