Thermoinactivation of Viruses: Report V. Thermoinactivation and Thermostabilizing Agents as a Factor Determining the Level of VEE Virus Production in Cell Cultures

Abstract

The general productivity of cells in mediums of different compositions was studied on the model of Venezuelan equine encephalomyelitis virus (VEE) and trypsinized chick embryo cells. Medium 199 with 2% bovine serum provided long-term and active virus production, the total yield of virus particles per one cell exceeding 10,000 PFU. In minimal essential medium (Earle's solution with 0,22% sodium bicarbonate) the total virus yield was more than 10 times lower despite the virus amplification to comparatively high titers. The most effective agents providing for adequate thermostabilization of VEE virus at 37 degrees (C) were found to be 2% bovine serum, 0,1% lactalbumin hydrolystate and 0,02% crystalline bovine albumin.

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Document Details

Document Type
Technical Report
Publication Date
Dec 02, 1971
Accession Number
AD0733265

Entities

People

  • A. S. Novokhatskii
  • F. I. Ershov
  • S. S. Girgorian

Tags

DTIC Thesaurus Topics

  • Additives (Chemicals)
  • Albumins
  • Arbovirus Infections
  • Calcium
  • Calcium Compounds
  • Chemistry
  • Chlorides
  • Culture Techniques
  • Infection
  • Interferon
  • Magnesium
  • Magnesium Compounds
  • Materials
  • Production
  • Virion
  • Viruses
  • Wound Infections

Fields of Study

  • Biology

Readers

  • Analytical Chemistry
  • Virology (or Medical Virology).