The Effect of DEAE-Dextran, Protamine Sulfate and Sulfated Polysaccharides of Agar on Multiplication of Venezuelan Equine Encephalomyelitis Virus in Cell Cultures

Abstract

The model of VEE virus and trypsinized chick embryo cells was used to study the effect of diethylaminoethyl-dextran (DEAE-D), agar polysaccharides (APS) and protamine sulfate (PS) on the pattern of virus-cell interaction. The presence in the growth medium of DEAE-D and PS inhibited virus production. The inhibiting effect of DEAE-D was manifested in the first stages of virus-cell interaction and was more marked with increasing pH. Sulfated polyanions of agar inhibited virus multiplication affecting later stages of reporduction. Lowering of pH of the growth medium activated the inhibiting effect of polysaccharides.

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Document Details

Document Type
Technical Report
Publication Date
Dec 02, 1971
Accession Number
AD0733637

Entities

People

  • A. S. Novokhatskii
  • F. I. Ershov
  • S. S. Grigoryan

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Aeration
  • Arbovirus Infections
  • Arboviruses
  • Cells
  • Chemical Properties
  • Coverings
  • Culture Techniques
  • Far East
  • Indicator Dyes
  • Infection
  • Inhibition
  • Polysaccharides
  • Production
  • Virion
  • Virology
  • Viruses
  • Wound Infections

Fields of Study

  • Biology

Readers

  • Molecular and Cellular Biochemistry
  • Virology (or Medical Virology).