Development and Substantiation of the Principles of Obtaining Large Amounts of Interferon

Abstract

The optimum conditions for the formation of interferon, induced by Group A arboviruses (VEE and Sindbis viruses), in primary trypsinized chick embryo fibroblast cultures, using various methods of cultivation (roller, and concentration of cells, (in terms of 1 ml of the medium), are the most important factors determining the level of interferon production. Multiplicity of infection is important, mainly when suspension is used, and is optimally 1 - 10 PFU.A rise in the incubation temperature brings about an increase in the production of interferon, induced by the Sindbis virus.

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Document Details

Document Type
Technical Report
Publication Date
Dec 07, 1971
Accession Number
AD0733824

Entities

People

  • A. S. Novokhatskii
  • F. I. Ershov

Tags

DTIC Thesaurus Topics

  • Arbovirus Infections
  • Cells
  • Incubation
  • Inductors
  • Infection
  • Interferon
  • Materials
  • Monomolecular Films
  • Mouth Diseases
  • Production
  • Proteins
  • Stationary
  • Tissue Culture
  • Venezuelan Equine Encephalomyelitis
  • Virology
  • Viruses
  • Wound Infections

Readers

  • Systems Analysis and Design
  • Virology (or Medical Virology).