Automated Quantitation of A and B Blood Group Substances.
Abstract
An automated assay for the detection and quantitation of soluble blood group antigens is described. Variables such as reaction medium, type and amount of antibody employed, and reaction temperature were investigated and optimal conditions for increased sensitivity, specifity, and precision were determined. This AutoAnalyzer system utilizing modified hemagglutination manifolds and standardized with an NIH 500 microgram/ml standard has the ability to detect and/or quantitate blood group substance A and B at the microgram level. Limitations of the assay are discussed. Rationale behind the need for screening parenteral fluids, especially in the military population is advanced. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 25, 1971
- Accession Number
- AD0735715
Entities
People
- Benjamin F. Meney Jr.
- C. Elliott Bell Jr.
- Frank R. Camp Jr.
- H. Philip Fortwengler Jr.
- Nicholas F. Conte
Organizations
- United States Army Medical Research Laboratory