'In vivo' Role of 'Pseudomonas aeruginosa' Toxins and Host Response

Abstract

Five standard methods of extracting endotoxin were employed in an effort to establish its presence in Pseudomonas aeruginosa as well as to make comparative evaluations of its biological and chemical properties. Of the five preparations, aqueous phenol extracted endotoxin exhibited the greatest degree of lethality. The LD50 was 450 micrograms dry weight when administered intravenously and 840 micrograms intraperitoneally. No lethality was observed when endotoxin was administered intranasally. Lethality appeared to be associated with the core region of the lipopolysaccharide molecule, while no correlation between lethality and lipid content was detected.

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Document Details

Document Type
Technical Report
Publication Date
Feb 29, 1972
Accession Number
AD0738063

Entities

People

  • John Dyke
  • Richard S. Berk

Organizations

  • Wayne State University

Tags

DTIC Thesaurus Topics

  • Amino Sugars
  • Carbohydrates
  • Cells
  • Cellular Structures
  • Chemical Composition
  • Ethers
  • Fatty Acids
  • Health Services
  • Hot Water
  • Hydrolysis
  • Materials
  • Molecules
  • Nucleic Acids
  • Ribonucleic Acids
  • Sugars
  • Test And Evaluation
  • United States

Readers

  • Molecular and Cellular Biochemistry
  • Toxicology/Environmental Toxicology
  • Trauma Surgery or Emergency Medicine.