Biochemical Studies on the Mechanism of Drug Action
Abstract
The effects of 5,5'diphenyl-2-thiohydantoin (DPTH) on mitochondria were examined in vitro and in vivo, on well delineated sites known to be profoundly affected by thyroid hormones, in order to test the hypothesis that thyroid hormones regulate metabolic responses through mitochondrial and cytosolic processes. The effect of methimidazole, another antithyroid drug, was studied in vitro in parallel systems. In vivo experiments where DPTH was added to the diets, a large inhibition of liver gluconeogenic enzyme activities, alpha-glycerophosphate dehydrogenase and oxygen consumption by rat liver tissue slices were observed. Acetylsalicylic acid feeding decreased the concentration of glycogen in liver, the activities of pyruvate carboxylase and phosphoenolpyruvate carboxykinase in liver, and the activity of phosphoenolpyruvate carboxykinase in kidney. The findings suggest that chronic acetylsalicylic acid ingestion may inhibit gluconeogeneisis by decreasing the activities of the regulatory enzymes.
Document Details
- Document Type
- Technical Report
- Publication Date
- Feb 01, 1972
- Accession Number
- AD0740802
Entities
People
- Myron A. Mehlman
Organizations
- University of Nebraska Omaha