Utilization of (32P) Soman for Measurement of Acetylcholinesterase in Brain Tissues

Abstract

Acetylcholinesterase (acetylcholine hydrolase, EC 3.1.1.7) activity in brain tissues of the dog was measured before and after inhibition with 32P- labeled pinacolyl methylphosphonofluoridate (Soman). A high correlation was obtained between uninhibited enzyme activity and soman-derived radiophosphorus bound to the tissues as methyl (32P) phosphonate. The methylphosphonate for 100% inhibition was taken equivalent to the catalytic sites. Turnover numbers calculated from the ratio of acetylcholine hydrolysis to catalytic sites approached values yielded by eel acetylcholinesterase.

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Document Details

Document Type
Technical Report
Publication Date
Apr 01, 1972
Accession Number
AD0741015

Entities

People

  • Joseph H. Fleisher
  • Larrel W. Harris
  • Norman C. Thomas

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Acetylcholinesterases
  • Animals
  • Biomedical Research
  • Brain
  • Cerebral Cortex
  • Chemical Warfare Agents
  • Confidence Limits
  • Enzymes
  • Experimental Data
  • Hydrolysis
  • Inhibition
  • Laboratory Animals
  • Measurement
  • Methylphosphonates
  • Phosphonates
  • Radioactivity
  • Substrates

Fields of Study

  • Biology

Readers

  • Analytical Chemistry
  • Mathematics or Statistics
  • Neuroscience