Quantitation of Spin Concentration of Some Free Radicals.
Abstract
A simple method for the quantitation of small free radicals has been developed using nitrosyl disulfonate and porphyrexide as model compounds. The double integral of the electron paramagetic resonance (EPR) signal of solutions of each free radical was obtained and correlated with optical density measurements. In this way, curves of normalized area (by EPR) versus concentration of radical were obtained and shown to be linear. Titrations of these radical solutions with dithiothreitol or alpha-thioglycerol caused a linear decrease in both the measured optical density and the measured doubly integrated EPR signals. A concept was developed called 'radical equivalency'. A reagent which provides n one-electron reducing sites per molecule has a 'radequivalency' equal to n times its molarity. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Dec 30, 1971
- Accession Number
- AD0741685
Entities
People
- B. David Polis
- Barbara J. Forman
- Walter Grobelney
Organizations
- Naval Air Warfare Center Warminster