Specificity of a Rabbit Antihuman Lymphocyte Serum.
Abstract
The specificity of antibodies produced after prolonged immunization of rabbits with purified human lymphocytes from a single individual (B.L.) was examined. Lymphocytes and leukocyte-platelet packs from B.L. and from ten other individuals unrelated to B.L. and to each other were used. The antiserum had a cytotoxicity titer of 1:256 against B.L. lympnocytes and against lymphocytes from eight other individuals. A titer of 1:128 was obtained against lymphocytes from the other two individuals. Absorption studies at 1:64 and 1:4 dilution of the antiserum indicated that separate absorptions with purified lymphocytes (1:64) or leukocyte-platelet packs (1:4) from each of the ten other individuals, as well as from B.L., completely removed cytotoxicity against both the absorbing lymphocytes and B.L. lymphocytes (with one exception at the 1:4 dilution). Absorption studies at the 1:64 dilution indicated that residual cytotoxicity existed for B.L. lymphocytes at the point where cytotoxicity was just removed against the absorbing lymphocytes from each of the ten other individuals. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 28, 1972
- Accession Number
- AD0741816
Entities
People
- C. Elliott Bell Jr.
Organizations
- United States Army Medical Research Laboratory