The Rapid Diagnosis of Acute Respiratory Disease Due to Adenovirus: Preparation and Standardization of Fluorescent Antibody Reagents

Abstract

Fluorescent antibody reagents were prepared for the rapid diagnosis of acute respiratory disease caused by adenovirus in a military population. Methods are described for the preparation of high-titered antisera in rabbits, fractionation of globulins from the serum, conjugation with fluorescein isothiocyanate, and sorption to remove nonspecific staining. Fluorescein-tagged antisera were specific at diluations of 1:20 to 1:30.

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Document Details

Document Type
Technical Report
Publication Date
May 01, 1972
Accession Number
AD0743082

Entities

People

  • Carl Berling
  • David P. Mccormick
  • Edward P. Smith
  • Quirino Galapon
  • Raymond S. Combs

Tags

DTIC Thesaurus Topics

  • Antibodies
  • Antigens
  • Blood Proteins
  • Chemistry
  • Diseases And Disorders
  • Health
  • Health Services
  • Immune Serums
  • Infection
  • Infectious Diseases
  • Microbiology
  • Navy
  • North Carolina
  • Proteins
  • Public Health
  • Standardization
  • Virology

Fields of Study

  • Biology
  • Medicine

Readers

  • Analytical Chemistry
  • Chemistry (specifically Chemical Fluorescence)
  • Virology (or Medical Virology).